How many cc’s of liquid culture or spores per injection port bag?

For efficient grows, use 2.5 ccs or mL per injection port bag. You can certainly use more per bag, which can speed up colonization. When working with spore syringes or liquid culture, the best sterile technique will use the entire syringe in one inoculation session. We do not recommend using an entire syringe in one injection port bag as it can be too much liquid in one container.